1D-ES-1: SDS SLAB GEL ELECTROPHORESIS. Option of 10, 12, 15, or 20 wells per comb. Includes weighing and dissolving samples; 2 lanes/sample. Example photo below shows food proteins. Lane 1, casein; 2, whey; 3, soy; 4, egg white; 5, whole egg; 6, beta-lactoglobulin; 7, alpha-lactalbumin; 8, bovine serum albumin; 9, IgG; 10, molecular weight markers.
Price: $145 for first sample; $70 per additional sample.
QUANTIFICATION OF PROTEINS RESOLVED BY 1-D (1D-ES-2). Requires 1D-ES-1. Includes scanning and quantitative analysis. Results are reported in a table showing % total stain density for each band in a gel lane (3 lanes/sample) plus printouts of lane density profiles and computer images. Note that the molar ratio of stain per protein may vary considerably from protein to protein. This is a good way to compare similar samples but not good for determining % protein by weight.
Price: Add $100 per sample.
MOLECULAR WEIGHT DISTRIBUTION PLOT (1D-ES-3). Requires 1D-ES-1. Includes scanning & analysis. Example Report
Price: Add $70 per sample.
DOUBLE THICKNESS (1.5mm) SLAB GELS (1D-ES-4) to maximize material loaded for transblotting and subsequent sequencing.
Price: Add $44 per gel.
PEPTIDE SLAB GEL (1D-ES-5) for resolution of low molecular weight (2000-12,000).
Price: Add $36 per gel.
Blue Native Polyacrylamide Gel Electrophoresis (1D-BN-PAGE) is a charge shift method for isolation and characterization of large multi-protein complexes (MPCs) in their native state. Novex Blue native minigels are used.
Price: $145 for first sample; $70 per additional sample.
Isoelectric focusing (1D-IEF) is useful for examining protein charge differences. Novex IEF minigels are used.
Price: $145 for first sample; $70 per each additional sample.
1-D ELECTROPHORESIS QUANTIFICATION PACKAGES. These packages are used to quantify proteins relative to a standard curve for determining percent of a known protein by weight in a sample. An example is the determination of percent casein and whey in dairy products. Each sample is weighed along with standards and dissolved by boiling in SDS buffer to a known concentration. A set of standards (4 concentrations in duplicate) is run with 2 concentrations of sample (in duplicate) on two gels. The gels are scanned, standard curves are generated, and the concentration of the unknown in the sample is determined from the standard curve. A report giving the concentration (for example casein percentage by weight) along with a description of the method is mailed out. Unless otherwise requested, dried gels are filed.
Price: 1D-PKG1: 1-2 samples, $360/sample; 1D PKG1A: 3-9 samples, $310/sample; 1D-PKG1B: 10 or more, $286/sample.
Percent Casein and Whey Protein by Weight.
Each sample is weighed and dissolved by boiling in SDS buffer to a known concentration. A set of standards (4 concentrations in duplicate using casein and whey standards) is run with 2 concentrations of sample (in duplicate) on two gels. The gels are scanned with a calibrated laser densitometer and the images analyzed using TotalLab software (TotalLab, Ltd) on a Windows 10 computer. Standard curves are generated and the concentration of casein and whey in the sample determined from the standard curves. A report (PDF) giving the concentration along with a description of the method is emailed and a printed copy mailed. Unless otherwise requested, dried gels are filed rather than sent to clients. See table below for prices.
Relative Amount of Whey Components.
Includes measurement of the Coomassie blue stain contained in the alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin, lactoferrin and immunoglobulin bands. The samples are analyzed by SDS slab gel electrophoresis in triplicate, the stained gels digitized with a laser densitometer, and computerized quantitative analysis of stain density of individual protein bands carried out. Results are presented in a table (see example report (PDF)) showing percent of total protein for each protein band (average of triplicate measurements) relative to total stain density per lane. Note: This method is best used for comparing similar samples since the stain/protein ratio may vary from protein to protein. It gives relative values rather that absolute percent protein by weight.
|Item #||Assay||Qualitative or Quantitative||Lower Limit of Sensitivity||Price Per Sample|
|1D-PKG-1||Percent any purified protein by weight; client must supply protein for standard curve if we don’t have it.||Quantitative||depends||1-2 $360
|1D-CW 1D-CW-A 1D-CW-B||Percent casein and whey by weight. Standard curves run for each.||Quantitative||<1.0%||1-2 each $210
3-9 each $165
>10 each $150
|1D-CPR 1D-CPR-A||Relative amounts of individual casein components. (stain density of α ,β and κ casein). Used to compare similar
|Quantitative but results expressed in relative terms||<0.5%||First $210
|1D-WPR 1D-WPRA||Relative amounts of individual whey components (stain density of α-lactalbumin, β-lactoglobulin, lactoferrin,
bovine serum albumin, and IgG bands). Used to compare similar samples.
|Quantitative but results expressed in relative terms||<0.01%||First $210
|1D-MPR 1D-MPR-A||Relative amounts of milk proteins (both casein and whey)||Quantitative but results expressed in relative terms||depends||First $210
|1D-TC 1D-TC-A||Trace casein detection by spiking known amounts of casein||Qualitative||< 1.0%||First $365
|1D-SP 1D-SP-A||Trace casein detection using electrophoresis + Western blotting||Qualitative||< 0.005||First $730
|1D-TCW 1D-TCW-A||Trace milk detection (casein and whey) using electrophoresis and western blotting||Qualitative||<0.005||First $830
|1D-ES-3 1D-ES-3A||Molecular weight distribution plot for hydrolyzed samples||Not applicable||N/A||First $215
|1D-EW 1D-EW-A||Egg white or whole egg detection||Qualitative||< 1.0%||First $360
|1D-LF 1D-LF-A 1D-LF-B||Lactoferrin by electrophoretic analysis. Standard curves are run. This assay is useful for powders.||Quantitative||<0.1%||1-2 $255
Table 1. Electrophoresis Assays Available for Food Proteins.
The sensitivities for these assays vary with interference and may be either greater or lower depending on the sample. Please call to get estimates for your particular sample type. Qualitative results are expressed in yes/no terms i.e. the protein being tested is either present or absent from the sample within the limits of sensitivity of the assay. More details