• Skip to primary navigation
  • Skip to main content
  • Skip to primary sidebar
  • Skip to footer

Three Decades of Experience in
Protein Contract Research
Request a Quote
  • Home
  • HCP Analysis
    • HCP Overview
    • Host Cell Protein (HCP) Antibody Analysis Method
    • Host Cell Protein (HCP) Results & Report
    • HCP Pricing
    • Product Characterization
    • Drug Substance Testing
  • 2D
    • 2D Overview
    • 2D Catalog
    • 2D Computer Comparisons
    • 2D Examples
    • Coomassie Blue Staining
    • Silver Staining
    • Standardization
    • Sample ID Form (PDF)
  • WB
    • 1D & 2D Western Blotting Services
    • HCP Analysis WB
    • Acetyl-lysine WB
    • P-Ser/P-Thr WB
    • P-Tyrosine WB
  • 1D
    • 1D Catalog
    • Food/Nutrition Testing
    • Sample ID Form (PDF)
  • Support
    • Sample Preparation
    • Accreditation
    • FAQs
    • Kendrick Labs Citations
    • White Papers/Recent Publications
    • Presentations
    • Misc
    • Terms & Conditions
  • Contact
    • Partnership Opportunities
    • Contact Us
    • How to Send Samples
    • Mission and People
    • Careers at Kendrick Labs
    • Sitemap

How Does 1D Gel Electrophoresis Work?

Schematic depicting how 1D SDS PAGE gel is run

Schematic representation of an
electrophoresis gel.

1D Electrophoresis is a method that separates protein by molecular weight over a range of about 10 to 300 kilodaltons (kDa). In this method, samples are weighed and dissolved in sodium dodecyl sulfate (SDS). SDS is a negatively charged detergent that has both hydrophilic (likes to associate with water) and hydrophobic regions (does not like to associate with water). SDS likes to bind to proteins with its hydrophobic region and also likes to be in water in its hydrophilic region. This SDS- protein-water interaction allows water insoluble proteins to dissolve in water, making SDS the best detergent to dissolve protein mixtures. The proteins are completely denatured (unraveled from their folded native structure) by the SDS. When an electric field is applied, the negative charge of the SDS causes the proteins to move through a clear acrylamide matrix toward the positive electrode. This matrix has holes in it that sieve out the proteins by molecular weight. Large proteins move more slowly through the matrix than the smaller proteins thereby separating proteins by molecular weight. Molecular weight standards are included on each gel to allow for determination of protein size.

Food/Nutrition Testing                     1D Pricing

Primary Sidebar

Contact Us

  800-462-3417
 2d@kendricklabs.com
 1202 Ann St, Madison, WI 53713

Key Links

  • 2D Services and Pricing
  • 1D Services and Pricing
  • Download Catalog (pdf)
  • 2D SDS Compatibility
  • Submit Samples
  • Sample Preparation

Klabs Email List

Loading

Footer

              

Copyright © 2025