Expert Western Blot Analysis Services

Since 1987, Kendrick Labs has been performing expert western blot analysis through 1D and 2D SDS PAGE for clients to identify proteins of interest. Western blotting continues to be the gold standard for detecting specific proteins in complex biological samples, and we have the experience you need.

  • Both 1D SDS PAGE and 2D SDS PAGE western blot analyses are available
  • Unique SDS compatible tube gels allow for superior resolution in 2D gels
  • Identification of proteins using LC-MS/MS services
  • Optimized methods for protein detection to the femtogram level
  • We offer a wide range of detection options including multiplexing and ECL
  • Capability for GMP/GLP projects

Western Blot service: specificity test of Anti-Goat IgG (H+L)-HRP secondary antibody. No cross-reaction seen with human IgG or rabbit IgG.

Example Image: Specificity test of our Anti-Goat IgG (H+L)-HRP secondary antibody. No cross-reaction seen with human IgG or rabbit IgG.

 

Experienced Protein Biochemists

  • Performing 1D and 2D SDS PAGE western blot services since 1987
  • Standardized methods with a robust infrastructure of SOP’s
  • Western blot conditions optimized for your project at no additional cost
  • Proactive and reactive QA division to ensure a standard of excellence

Detection Methods

Enhanced Chemiluminescence Fluorescence Colorimetric
Detection Reagents Pierce ECL, ECL Prime Cyanine Cyes (Cy3, Cy5) NBT/BCIP
Sensitivity Best (picogram to femtogram) Good (femtogram to nanogram) Least (nanogram)
Imaging X-ray Film (Linear Scanner)

ChemiDoc XRS+

 Typhoon FLA9000 Flatbed Scanner
Quantitative Yes Yes No
Multiplexing Possible with re-probing Yes, can be done simultaneously No

Wide Variety of Electrophoresis Options

  • 2D SDS PAGE Gels, In-house
    • Gel Size: Standard Format (13 x 15 cm) or Large Format (22 x 20 cm)
    • IEF dimension pH ranges: 3-10, 4-8, custom mix available
    • Straight percentage acrylamide: 7%, 10%, 12% 16.5%, custom available
    • Buffer system: Tris-Glycine, Tris-Tricine
  • 1D SDS PAGE Gels
    • Gel Size: Standard Format (13 x 15 cm) or Large Format (22 x 20 cm)
    • Straight percentage acrylamide: 7%, 10%, 12% 16.5%, custom available
    • Buffer system: Tris-Glycine, Tris-Tricine
  • Commercially available mini gels from ThermoFisher (Novex)
    • Gel Size: 8 x 8 cm
    • Large variety of different percentage acrylamide including gradient gels and buffers
    • Native 1D gels and IEF 1D gels available

Reversible Total Protein Stain

Our PVDF membranes are stained with Kendrick Labs proprietary Coomassie blue (CB) method to detect total protein:

  • Completely reversible staining method is fully compatible with western blotting
  • The Coomassie blue-stained membrane image is superimposable with the western blot
  • Allows for easy matching of proteins to CB gels for Mass Spectrometry or silver gels for Host Cell Protein analysis
  • The total protein stain ensures equalized sample loading and transfer efficiency

PTM Blotting Packages

Kendrick Labs offers PhosphoTyrosine, PhosphoSerine/Threonine, and Acetyl-Lysine Western blotting packages at a discounted rate. These packages use optimized in-house antibodies which are included in the price.

Questions for Us?

Contact our scientists! Call us: 800-462-3417