Western blotting has many uses by enabling researchers to look at a single protein or subset of proteins on complex 2D gels with very high sensitivity via antibody binding. For example, Western blotting may be used to follow protein phosphorylation. An antibody that binds to all the isoforms of a poly-phosphorylated protein will show a … Continue reading 2D Gel Western Blotting Protein & Electrophoresis